Nami In the present koenigi, we report the antibacterial activities of the ethanol extract of M. These results shows that the root extract chloroform, Petroleum ether can be used to formulate antimicrobial compositions. Murraya koenigii Linn, Spreng. Modified agar diffusion method 7 was adopted for this study. The extractive values for petroleum ether, chloroform, ethyl acetate and ethanol extracts was found to be 4.
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This article has been cited by other articles in PMC. Abstract Objective: This study is designed to extract and examine chemical composition, antimicrobial and antioxidant activity of the hydro-distillated essential oil of Murraya koenigii leaves from the south region of Tamilnadu, India. Results: Major compounds detected in the oil were Linalool From the identified compounds, they were classified into four groups that are oxygenated monoterpenes The antibacterial activity of essential oil has pronounced by Disc Diffusion Method against various pathogenic microbes.
Conclusion: The oil has a maximum zone of inhibition ability against Corynebacterium tuberculosis, Pseudomonas aeruginosa, Streptococcus pyogenes, Klebsiella pneumonia and Enterobacter aerogenes. The antioxidant profile of the sample was determined by different test systems. In all the systems, essential oil showed a strongest activity profile within the concentration range. Key Words: Antioxidant activity, Chemical composition, Essential oil, Murraya koenigii L leaves Introduction Aromatherapy is a form of alternative medicine that uses volatile plant materials.
In nature, it plays an important role in the protection of the plants as antibacterial, antiviral, antifungal and insecticides. Many oils show antibacterial, fungicidal, relaxing, stimulating, antidepressant effect and may be a very effective therapeutic agent. Essential oils are known for their therapeutic properties hence, used in the treatment of various infections caused by both by pathogenic and non-pathogenic diseases Hamid et al.
Essential oils have been traditionally used for 35 differtent respiratory tract infections, and are used now a day as ethical medicines for colds. Essential oils and their individual aroma components also showed cancer suppressive inactivity when tested on a number of human cancer cell lines including glioma, tumors, breast cancer, leukemia and others Hamid et al. In the recent decades, there has been increasing interest in the use of plant antioxidants for scientific research as well as industrials purpose.
Therefore, the need exists for safe, economical, powerful and natural antioxidants to replace synthetic ones Tadhani et al. Antioxidants obtained from natural plant sources are more potent and safe due to their harmless nature. Medicinal plant and wild herbs are always under investigation due to these facts. Shahidi et al. The antioxidant ability of different plant extracts and oils can be measured using numerous assays. The various tested are based on specific features of the antioxidant activity Chu et al.
Murraya koenigii L. Spreng or its common name curry leaf tree is a small strong smelling perennial shrub commonly found in forests as undergrowth. It was originally cultivated in India for its aromatic leaves and for ornament is normally used for natural flavoring in curries and sauces. Originated in Tarai regions of Uttar Pradesh, India. It is now widely found in all parts of India and it adorns every house yard of southern India and also it is now cultivated and distributes throughout the world.
The plant is used in Indian system of medicine to treat various ailments Kumar et al. Parts of the plant have been used as raw material for the traditional medicine formulation in India. This plant is known to be the richest source of carbazole alkaloids. It has been reported by authors that carbazole alkaloids present in M. Spreng and display various biological activities such as anti-tumor, anti-oxidative, anti-mutagenic and anti-inflammatory activities Chakravarthy et al.
The aromatic leaves, which retains their flavor and other qualities even after drying, are slightly bitter, acrid, cooling, weakly acidic in tastes and are considered as a tonic, anthelmintic, analgesic, digestive, appetizing and are widely used in Indian cookery for flavoring food stuffs Adebajo et al. The phytoconstituents isolated so far from the leaves are alkaloids viz. Essential oil composition of the leaves has been studied by various workers Rana et al.
This essential oil from M. The increasing importance of essential oils as pharmaceutical and aromatherapy assist besides their traditional role in cosmetics, not only as a potent ingredient but also as a fragrance donor. Moreover, it has opened up world wide opportunities for global marketing. As far as our literature survey could ascertain, the selected plant essential oils from M. The purpose of this study was to evaluate essential oil of Murraya koenigii leaves as a new potential source of natural antioxidants and phenolic compounds.
Materials and Methods Isolation of the essential oil The fresh leaves of M. The columns were 30 m x 0. Antibacterial activity In the recent years due to an upsurge inantibiotic-resistant infections, the search for novel bioactive compounds to fight infections is an absolute necessity and in this regard, plant essential oils may offer a great potential and hope. In this study, the essential oil was tested against ten bacteria, including Gram positive and gram-negative. A sterile 5-mm diameter filter paper disk Whatman paper no.
A negative control was also included in the test using a filter paper disk 5mm size saturated with DMSO dimethyl sulfoxide to check possible activity of this solvent against the bacteria assayed.
The antibacterial activity was assessed by measuring the zone of growth inhibition size in mm surrounding the disks. Each experiment was carried out in triplicate. Antioxidant profile of essential oil Scavenging effect on 2,2-diphenylpicrylhydrazyl radical DPPH The radical scavenging capacity was determined according to the method described by Mensor et al.
The samples were kept at room temperature in the dark and after 30 min the optic density was measured at nm. The optic density of the samples, the control and the empty samples were measured in comparison with ethanol. Detection of hydroxyl radicals by deoxyribose assay The assay was performed as described elsewhere Halliwell et al.
All solutions were freshly prepared. After cooling, the absorbance was measured at nm against a blank containing only buffer and deoxyribose. Quercetin was used as a positive control. Assay of Superoxide Radical Anion Superoxide anions were generated in an enzymatic system xanthine-xanthine oxidase and assayed by the reduction of nitroblue tetrazolium.
The mixture was homogenized for 5 min according to Yen et al. The antioxidant was added at the final concentrations of 0, 0. A higher percentage indicates a higher antioxidative activity. Determination of conjugated diene CD formation Aliquots of 0. Determination of thiobarbituric acid reactive substances A modified thiobarbituric acid reactive substances TBARS method was used to measure the antioxidant activity of the essential oil in terms of inhibition of lipid peroxidation.
After 2 ml of chloroform were added, the mixture was mixed and centrifuged at rpm for 15 min. The chloroform layer was separated and the absorbance of the supernatant was measured at nm against a blank containing TBA-TCA solution.
For all cases the plural correlation coefficient R2 was determined. Results The essential oil of Indian curry Murraya koenigii leaf collected from Kanyakumari district South Tamilnadu, India has been investigated for its composition and is responsible for the aroma and flavor associated with herbs, spices, and perfumes and strong antibacterial and antifungal activity when tested with microorganisms. The oils from the curry leaves were found to contain mostly oxygenated monoterpenes.
From these identified compounds are classified in to 4 groups that are monoterpene hydrocarbons
Received Nov 29; Accepted May This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. This article has been cited by other articles in PMC. Associated Data The data used to support the findings of this study are available from the corresponding author upon request. Abstract Development of multidrug resistance among pathogens has become a global problem for chemotherapy of bacterial infections.
ANTIBACTERIAL ACTIVITY OF MURRAYA KOENIGII PDF
This article has been cited by other articles in PMC. Abstract Objective: This study is designed to extract and examine chemical composition, antimicrobial and antioxidant activity of the hydro-distillated essential oil of Murraya koenigii leaves from the south region of Tamilnadu, India. Results: Major compounds detected in the oil were Linalool From the identified compounds, they were classified into four groups that are oxygenated monoterpenes The antibacterial activity of essential oil has pronounced by Disc Diffusion Method against various pathogenic microbes. Conclusion: The oil has a maximum zone of inhibition ability against Corynebacterium tuberculosis, Pseudomonas aeruginosa, Streptococcus pyogenes, Klebsiella pneumonia and Enterobacter aerogenes.