GERBERA MICROPROPAGATION PDF

Brat However, in this study, at a very high concentration of BAP and other growth regulators, development and growth of shoots were inhibited. Numerous types of explants and protocols for micropropagation have been established and used for gerbera. Rooted plantlets of Gerbera were successfully established and all plantlets were transferred to soil and maintained in the green house. Cytokinin alone in the culture medium induces shoot formation in many plants. In Mocropropagation jamesoniiBAP was also required for the formation of callus.

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However, white, creamy friable callus was obtained when explants were cultured on MS medium supplemented with 2, 4-D alone. Gerbera jamesonii is also used in the preparation of traditional Chinese medicine, tu-er-feng, for curing cold and also for treating rheumatism Ye et al. Micropropagation is the main system used to clonally propagate gerbera in vitro resulting in the production of millions of plantlets each year.

Micropropagation of Gerbera Gerbera jamesonii Bolus. Principles of Plant Biotechnology. One of the most important techniques in plant improvements is via micro propagation. Petiole segment was identified as the best explant for regeneration of this species. On Tu-er-fang derived from Gerbera plants.

The propagation rates via organogenesis can be much higher than axillary shoot proliferation Chun, The use of an in vitro adventitious bud micropropgaation for mutation breeding of Begoniaxhiemalis. This technology is being utilized commercially in the ornamentals industry and in other plant production organizations worldwide Chu, ; Huetteman and Preece, ; Mantell et al.

Despite this, no successful universal protocol has yet been developed for multiple cultivars, limiting the usefulness of current protocols for commercial biotechnology labs. Capitulum explant promoted callus development. The authors wish to thank the Institute of Biological Sciences and University of Malaya, Kuala Lumpur, Malaysia for providing all facilities and financial support. However, regeneration of root was not observed when explants were cultured on MS medium supplemented with BAP and 2, 4-D.

Gerbera jamesonii gerbera is an important cut-flower in the global floricultural industry. Successful in vitro propagation of ornamental plants is now being widely used in commercialization purposes. Shoots were obtained after 28 days of culture and shoot growth was normal. Plantlets survival rate achieved was Growth Optimization and Organogenesis of Gerbera jamesonii Bolus ex.

Many commercial ornamental plants are being propagated by in vitro culture on the culture medium containing auxins and cytokinins Preil, ; Rout and Jain, Various explants have been used for direct shoot formation. In vitro Culture of Higher Plants. Hook by tissue culture technique. Relatively, in this experiment, callus formation was optimum when leaf explant was cultured on MS medium supplemented with 1. Combination of auxin and cytokinin induces the formation of adventitious shoots and roots.

BAP strongly enhanced regeneration of shoots in petiole explants of Gerbera jamesonii. In vitro plantlet production from young capitulum explants of Gerbera jamesonii. Shoots and roots of chrysanthemum were developed on a single medium containing 1. High concentrations of cytokinin in culture medium were found to be unsuitable for shoot formation from leaf or petiole explants in some ornamentals. Finally the seeds were rinsed 3 times with sterile distilled water. In Gerbera jamesoniiBAP was also required for the formation of callus.

However, higher addition of auxin compared to cytokinin in the culture medium resulted in the inhibition of shoot formation. There was a problem providing the content you requested Epigenetic variations in micropropagated gerbera are frequently observed only with high concentrations of cytokinins in the culture medium but somaclonal variation micropropagtaion rare. Kluwer Academic Publishers, Dordrecht, pp: Thus, improvements of plant quality need to be studied and more research needs to be done.

Lower concentration of TDZ promoted direct shoot regeneraration and higher concentrations promoted callus induction in Hagenia abyssinica Bruce J. Callus with good and compact structure was obtained when explant was cultured on MS medium supplemented with the combination of BAP and 2, 4-D. Micropropaagation explants were found to be non-regenerative in all experiments conducted.

Adventitious shoots were obtained from petiole explants cultured on Murashige and Skoog MS medium supplemented with 2. Rosaceae from leaf explants. Related Posts

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Gerbera micropropagation

Mezitilar However, in this study, at a very high concentration of BAP microproapgation other growth regulators, development and growth of shoots were inhibited. Gerbera jamesonii is also used in the preparation of traditional Chinese medicine, tu-er-feng, for curing cold and also for treating rheumatism Ye et al. Studies of Gerbera clonal propagation could also be efficiently adapted for other ornamental plants. The first step mivropropagation de novo regeneration is to establish callus or cell suspension cultures.

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GERBERA MICROPROPAGATION PDF

However, white, creamy friable callus was obtained when explants were cultured on MS medium supplemented with 2, 4-D alone. Gerbera jamesonii is also used in the preparation of traditional Chinese medicine, tu-er-feng, for curing cold and also for treating rheumatism Ye et al. Micropropagation is the main system used to clonally propagate gerbera in vitro resulting in the production of millions of plantlets each year. Micropropagation of Gerbera Gerbera jamesonii Bolus. Principles of Plant Biotechnology. One of the most important techniques in plant improvements is via micro propagation.

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Micropropagation of Gerbera (Gerbera jamesonii Bolus).

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